Leaves are collected in Eppendorf, immediately freezed in liquid nitrogen after collection, then lyophylised. Eppendorf were then labelled with the identity code present in Gene Bank database
Populus nigra
Dry juvenile leaves
2009
Leaves are collected and stored in eppendorf (in natural conditions). They are, as quickly as possible, freezed in liquid nitrogen (cryogenic storage tank in the car) and stored in the evening (back at the office) at -80°C, then lyophylised. Eppendorfs were then labelled with the identity code present in Gene Bank database
Populus nigra
Dry juvenile leaves
2010
Populus trichocarpa x Populus deltoides
Juvenile leaves of poplar genotypes
2008
Leaves are collected in Eppendorf, immediately freezed in liquid nitrogen after collection, then lyophylised. Eppendorf were then labelled with the identity code present in Gene Bank database